Journal: bioRxiv

Article Title: Novel Transgenic Humanized Alpha-1 Antitrypsin Deficiency Mouse Model on Murine SERPINA1 Null Background

doi: 10.1101/2024.10.11.617858

Figure Lengend Snippet: (A) Transmission electron microscopy (TEM) images of a hepatocyte from Pi*M mouse liver tissue (n=1) and (B) Pi*Z mouse (n=1). LD: lipid droplet. (C) Space of Disse in Pi*M liver tissue as compared to (D) Pi*Z. Black arrow indicates collagen deposition in the space of Disse from Pi*Z mouse liver tissue. (E) Endoplasmic reticulum and mitochondrial morphology of the Pi*M as compared to (F) Pi*Z liver tissue. Black arrows indicate endoplasmic reticulum of the Pi*Z liver section. (G) The expression levels of VDAC1 and GRP75 in liver homogenates of Pi*M and Pi*Z mice as assessed by western blotting. (H) Quantification of the band intensities of VDAC1 and GRP75. (I) Western blot analysis on liver tissue lysates determining the expression levels of BiP, CHOP, heat shock protein 60 (Hsp60), and ATF4 in the liver of Pi*Z as compared to Pi*M mice (n=3). p-values have been calculated using student t-test method. Pi*M, normal variant of human α-1 -antitrypsin; Pi*Z, mutant human α-1 -antitrypsin.

Article Snippet: GRP75, VDAC1, HSP60, ATF4, and GAPDH rabbit anti mouse primary antibodies were obtained from ProteinTech (Rosemont, IL).

Techniques: Transmission Assay, Electron Microscopy, Expressing, Western Blot, Variant Assay, Mutagenesis

Journal: Molecular cell

Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α

doi: 10.1016/j.molcel.2023.05.008

Figure Lengend Snippet: Figure 5C

Article Snippet: Mouse Monoclonal Anti-CREB (86B10) , Cell Signaling Technology , Cat. # 9104S; RRID:AB_490881.

Techniques: Control, Virus, Recombinant, Expressing, Protease Inhibitor, Lysis, Transfection, Electron Microscopy, Immunodepletion, Cloning, Protein Purification, Endotoxin Assay, Bradford Assay, Staining, Mass Spectrometry, Mutagenesis, Software, Isolation

Journal: Cell Reports Methods

Article Title: A fluorescent splice-switching mouse model enables high-throughput, sensitive quantification of antisense oligonucleotide delivery and activity

doi: 10.1016/j.crmeth.2023.100673

Figure Lengend Snippet:

Article Snippet: Human embryonic kidney 293 (HEK293) cells were cultured in DMEM high glucose supplemented with 10% FBS and 1% P-S. HEK293 cells stably expressing a nuclear localized Cre recombinase (SC004-Puro, GenTarget Inc.) were cultured in DMEM high glucose supplemented with 10% FBS, 1% P-S, 2 mM L-glutamine (Gibco), and non-essential amino acids (Gibco) as suggested by the vendor.

Techniques: Recombinant, Electron Microscopy, Ligation, Sequencing, Expressing, Software, Imaging

Journal: Cell Metabolism

Article Title: Hypothalamic pregnenolone mediates recognition memory in the context of metabolic disorders

doi: 10.1016/j.cmet.2021.12.023

Figure Lengend Snippet:

Article Snippet: Rabbit Phospho-CREB (Ser133) (87G3) mAB , Cell Signaling , Cat# 9198S; RRID: AB_2561044.

Techniques: Virus, Recombinant, Electron Microscopy, Saline, Enzyme-linked Immunosorbent Assay, Stripping Membranes, Reverse Transcription, RNAscope, Hybridization, Software, Western Blot